Characterization of Brucella abortus Soluble Antigen Employed in Immunoassay

Characterization of Brucella abortus soluble antigen employed in immunoassay.

A soluble antigen extract of Brucella abortus (BASA) has been prepared by the National Veterinary Services Laboratories and furnished to a number of workers who are examining antibody-mediated and cell-mediated immune responses of cattle infected with B. abortus. Three lots of BASA were examined. There were quantitative but not qualitative differences among lots by content of protein, total car...

Structure and serology of the Brucella abortus O-antigen.

Cytokine response of T-cell subsets from Brucella abortus-infected mice to soluble Brucella proteins.

Hot saline extracts of Brucella abortus 19 were separated by successive differential precipitation with 50 and 70% ammonium sulfate, yielding fractions SBP50, with predominantly 36-kDa proteins and a number of medium-sized proteins (26 to 33 kDa), and SBP70, with 14-kDa and lower-molecular-mass proteins. Both fractions stimulated specifically proliferation and cytokine production by spleen cell...

Antigens of Brucella abortus. I. Chemical and immunoelectrophoretic characterization.

Extracts of Brucella abortus 2308S, prepared either by aqueous extraction of sonically ruptured cells or by phenol-water extraction of whole cells, were subjected to various fractionation procedures and then analyzed to determine their immunoelectrophoretic patterns and chemical properties. Fraction A, prepared from sonic extracts, contained at least nine precipitable components when analyzed b...

Genetic characterization of Brucella melitensis and Brucella abortus geographical clusters in Italy.

The genetic diversity of Brucella melitensis and Brucella abortus strains isolated in 199 cattle and sheep from 156 brucellosis outbreaks which occurred in 8 regions of Southern Italy in 2011, was determined using a Multiple-Locus Variable Number of Tandem Repeats Analysis approach. The existence of possible genetic clusters was verified through a hierarchical cluster analysis based on 'single ...